Recombinant Bovine HRH1 Protein, His/FLAG-tagged

• Cat.No.: HRH1-782B
• Product Overview: Recombinant Bovine Histamine H1-receptor and Regulator of G-protein Signaling 4 Protein (NM_174083) was ao-expressed in Sf9 insect cells.

Specification

• Species: Bovine
• Source: Insect Cells
• Tag: Flag&His
• Description: The histamine H1-receptor is expressed in numerous cell types and couples to Gq-proteins to activate phospholipase C. In Sf9 insect cells, bH1R couples to the endogenous insect cell Gq-protein. Co-expression with RGS proteins facilitates detection of high-affinity GTPase activity. bH1R reconstituted with RGS4 was prepared from Sf9 cells coinfected with bH1R- and RGS4-encoding baculoviruses. bH1R contains a N-terminal FLAG-tag™ and a C-terminal hexahistidine (His6)-tag for immunological detection, to allow purification and to provide additional protection against proteolysis.
• Form: 75 mM Tris-HCl pH 7.4, 12.5 mM MgCl2 and 1 mM EDTA
• Molecular Mass: 60 + 25 kDa
• Storage: Store at -80 centigrade, avoid freeze/thaw cycles.

Gene Information

• Gene Name: HRH1 histamine receptor H1 [ Bos taurus (cattle) ]
• Official Symbol: HRH1
• Synonyms: HRH1
• Gene ID: 281231
• mRNA Refseq: NM_174083.4
• Protein Refseq: AAI40476.1
• UniProt ID: P30546