Recombinant Rat RHRH1 Protein, His/FLAG-tagged

• Cat.No.: HRH1-787R
• Product Overview: Recombinant Rat Histamine H1-Receptor and Regulator of G-protein Signaling 4 was co-expressed in Sf9 insect cells.

Specification

• Species: Rat
• Source: Insect Cells
• Tag: Flag&His
• Description: The histamine H1-receptor is expressed in numerous cell types and couples to Gq-proteins to activate phospholipase C. In Sf9 insect cells, rH1R couples to the endogenous insect cell Gq-protein. Co-expression with RGS proteins facilitates detection of high-affinity GTPase activity. rH1R reconstituted with RGS4 was prepared from Sf9 cells coinfected with rH1R- and RGS4-encoding baculoviruses. rH1R contains a N-terminal FLAG-tag and a C-terminal hexahistidine (His6)-tag for immunological detection, to allow purification and to provide additional protection against proteolysis.
• Form: 75 mM Tris-HCl pH 7.4, 12.5 mM MgCl2 and 1 mM EDTA
• Molecular Mass: 30 + 25 kDa
• Storage: Store at -80 centigrade, avoid freeze/thaw cycles.

Gene Information

• Gene Name: Hrh1 histamine receptor H 1 [ Rattus norvegicus ]
• Official Symbol: HRH1
• Synonyms: Hisr; HRH1
• Gene ID: 24448
• mRNA Refseq: NM_017018.1
• Protein Refseq: NP_058714
• UniProt ID: P31390