Recombinant Borrelia afzelii flaB Antigen, His&TrxA tagged

• Cat.No.: flaB-018B
• Product Overview: The central region of the Borrelia afzelii flaB gene has been expressed as a recombinant antigen fused to a TrxA and a his-tag in its N-terminus.

Specification

• Species: Borrelia afzelii
• Source: E.coli
• Tag: N-His&TrxA
• Description: Flagellin is the subunit protein that polymerizes to form the filaments of bacterial flagella. It consists of 336 amino acids. Borrelia flagellin contains conserved and semi-conserved regions located at the N- and C-termini of the protein, whereas the central area exhibits variability. Many Borrelia-specific epitopes were found in this variable central region, and therefore, it has been described as a suitable antigen to test bacterial genera.
• Tag: N-His&TrxA
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 25 and 35 kDa, while relative molecular mass calculated from amino acid sequence is 25,625.57 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 7, 0.15 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.16 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Jiang, W. Luft, B.J., Schubach, W., Dattwyler, R.J. and P.D. Gorevic. Mapping the Major Antigenic Domains of the Native Flagellar Antigen of Borrelia burgdorferi. 1992, Journal of Clinical Microbiology, 30:1535-1540.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.