Recombinant B. burgdorferi P27 Protein, MBP-tagged

• Cat.No.: P27-10B
• Product Overview: Recombinant Borrelia burgdorferi Surface Lipoprotein P27 protein produced in E. coli. Protein contains a MBP-tag and is visible on SDS-PAGE at 73.3 kDa for P27-MBP (30.9 kDa for P27 and 42.4 kDa for MBP).

Specification

• Species: Borreliella burgdorferi B31
• Source: E.coli
• Tag: MBP
• Description: Surface Liporotein P27 is encoded by the spirochete B. burgdorferi, which is carried by Ixodes ticks. Strain B31 is the type strain (ATCC 35210) for this organism and was derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour. Spirochetal outer surface proteins (Osps) play a major role in both protection from and pathogenesis of Lyme disease. All Osps (OspA to OspF and P27) have been shown to be lipoproteins, containing hydrophobic N-terminal domains and putative cleavage sites (Leu-X-Y-Z-Cys) thought to be recognized by a lipoprotein signal peptidase. The p27 gene is located on a linear plasmid of a size of approximately 55 kb and expressed as a basic protein of 248 amino acids with a typical prokaryotic leader sequence of 17 amino acid residues at the N-terminus of the proposed translation product, as confirmed by Northern and Western blot analysis).
• Form: Liquid, 0.2 μm filter sterilised.
• Molecular Mass: 73.3 kDa for P27-MBP (30.9 kDa for P27 and 42.4 kDa for MBP)
• Purity: > 90%
• Storage: Short term: +2 to +8 centigrade
  Long Term: -80 centigrade
  Can be frozen, but avoid multiple freeze/thaw cycles
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide.

Gene Information

• Official Symbol: P27
• Synonyms: P27