Recombinant B. burgdorferi OspA Protein, MBP-tagged

• Cat.No.: OspA-07B
• Product Overview: Recombinant Borrelia burgdorferi OspA protein produced in E. coli. Protein contains a MBP-tag and is visible on SDS-PAGE at 70.5 kDa for OspA-MBP (28.1 kDa for OspA, 42.4 kDa for MBP alone kDa).

Specification

• Species: Borreliella burgdorferi B31
• Source: E.coli
• Tag: MBP
• Description: Outer-Surface Protein A (OspA) is a 31 kDa lipoprotein encoded by Borrelia burgdorferi and is a major component of the spirochete’s extracellular matrix, probably associated with lipid rafts and serving as a lipid-anchor (Toledo, et al., 2014). Strain B31 is the type strain (ATCC 35210) for this organism and was derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour. The Borrelia species causing Lyme disease express different OspA serotypes on their surface, B. burgdorferi (serotype 1), B. afzelii (serotype 2), B. garinii (serotypes, 3, 5 and 6) and B. bavariensis (serotype 4).
• Form: Liquid, 0.2 μm filter sterilised.
• Molecular Mass: 70.5 kDa for OspA-MBP (28.1 kDa for OspA, 42.4 kDa for MBP alone kDa)
• Purity: > 90%
• Storage: Store vial at -20 centigrade prior to opening. Aliquot contents and freeze at -20 centigrade or below for long term storage. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
  Can be frozen, but avoid multiple freeze/thaw cycles.
• Concentration: 1.04 mg/mL by UV absorbance at 280 nm
• Storage Buffer: In 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide.

Gene Information

• Official Symbol: OspA
• Synonyms: OspA; OSPA; outer surfce protein A
• Protein Refseq: ZP_03087263