Recombinant B. burgdorferi DbpA Protein, MBP-tagged

• Cat.No.: DbpA-03B
• Product Overview: Recombinant Borrelia burgdorferi DbpA protein produced in E. coli. Protein contains a MBP-tag and is visible on SDS-PAGE at 60.9 kDa for DbpA-MBP, (18.5 kDa for DbpA and 42.4 kDa for MBP)

Specification

• Species: Borreliella burgdorferi B31
• Source: E.coli
• Tag: MBP
• Description: B. burgdorferi sensu stricto has a linear plasmid (lp54) which carries a two-gene operon encoding two surface lipoproteins, DbpA and DbpB, both of which bind Decorin Binding Protein A (DbpA). It should be noted that other microbial DbpA’s, such as E. coli (ATP-dependent RNA helicase DbpA), are significantly different to Borrelia DbpA. DbpA and DbpB are surface-exposed outer membrane lipoproteins that mediate the attachment of Borrelia to decorin, a major component of the host extracellular matrix, enabling bacteria to colonize mammalian tissues. Both can mediate interaction with the glycosaminoglycans (GAGs) heparin and dermatan sulfate, but only DbpB binds chondroitin sulfate.
• Form: Liquid, 0.2 μm filter sterilised.
• Molecular Mass: 60.9 kDa for DbpA-MBP, (18.5 kDa for DbpA and 42.4 kDa for MBP)
• Purity: > 90%
• Storage: Short term: +2 to +8 centigrade
  Long Term: -80 centigrade
  Can be frozen, but avoid multiple freeze/thaw cycles
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide.

Gene Information

• Gene Name: dbpA decorin-binding protein DbpA [ Borreliella burgdorferi B31 ]
• Official Symbol: dbpA
• Synonyms: dbpA; decorin-binding protein DbpA
• Gene ID: 1194347
• Protein Refseq: WP_010890380