Recombinant B. burgdorferi CspA Protein, MBP-tagged

• Cat.No.: CspA-01B
• Product Overview: Recombinant Borrelia burgdorferi CRASP-1 protein produced in E. coli. Protein contains a MBP-tag and is visible on SDS-PAGE at 69.3 kDa for CRASP-1-MBP (26.9 kDa for CRASP-1 and 42.4 kDa for MBP)

Specification

• Species: Borreliella burgdorferi B31
• Source: E.coli
• Tag: MBP
• Description: Complement Regulator-Acquiring Surface Protein 1 (CRASP-1, CspA) is a 25.9 kDa multifunctional protein, encoded by the spirochete B. burgdorferi, which is carried by Ixodes ticks. Strain B31 is the type strain (ATCC 35210) for this organism and was derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour. Native CRASP-1 represents a homodimer with a previously unknown complex protein fold. CRASP-1 and four additional immune evasion proteins bind combinations of human plasma regulators, including collagen I, collagen III, collagen IV, fibronectin, laminin, factor H-like protein 1 (FHL-1) (resulting in inhibition of complement activation in mammals) and Human Bone Morphogenic Protein 2. These interactions may contribute to adhesion, bacterial colonization, and organ tropism to promote dissemination of B. burgdorferi in the host.
• Form: Liquid, 0.2 μm filter sterilised.
• Molecular Mass: 69.3 kDa for CRASP-1-MBP (26.9 kDa for CRASP-1 and 42.4 kDa for MBP)
• Purity: > 90%
• Storage: Short term: +2 to +8 centigrade
  Long Term: -80 centigrade
  Can be frozen, but avoid multiple freeze/thaw cycles
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide.