Recombinant Active Human TMPRSS2 Protein (107-492), N-FLAG and C-Myc/His-tagged

• Cat.No.: TMPRSS2-13H
• Product Overview: The recombinant protein contains the three extracellular domains (LDLRA, SRCR, serine protease) of TMPRSS2 with a C-terminal His(6)-tag. The target sequence begins with Lys107 and ends with Gly492. The protease is in its activated form, which means that the peptide bond between Arg255 and Ile256 is cleaved.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His&Myc
• Protein Length: 107-492
• Description: This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. This protein also facilitates entry of viruses into host cells by proteolytically cleaving and activating viral envelope glycoproteins. Viruses found to use this protein for cell entry include Influenza virus and the human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Bio-activity: Recombinant human TMPRSS2 cleaves the synthetic substrate Z-Gly-Gly-Arg-AMC with high efficiency (kcat/KM=2×10^5 M-1s-1). Its natural inhibitor HAI-2 completely blocks the TMPRSS2 activity at 1:1 molar ratio.
• Molecular Mass: 50 kDa (including tags)
• AA Sequence: KFMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPVCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGESALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKLQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDSGGPLVTSKNNIWWLIGDTSWGSGCAKAYRPGVYGNVMVFTDWIYRQMRADG
• Purity: More than 90% as determined by SDS-PAGE.
• Storage Buffer: 0.15 mg/mL TMPRSS2 in 20mM HEPES, pH 7.4, 150mM NaCl.

Gene Information

• Gene Name: TMPRSS2 transmembrane serine protease 2 [ Homo sapiens (human) ]
• Official Symbol: TMPRSS2
• Synonyms: TMPRSS2; transmembrane serine protease 2; PRSS10; transmembrane protease serine 2; epitheliasin; serine protease 10; transmembrane protease, serine 2; EC 3.4.21.-
• Gene ID: 7113
• mRNA Refseq: NM_005656
• Protein Refseq: NP_005647
• MIM: 602060
• UniProt ID: O15393