Recombinant Active Human ROS1 (L2086F) Protein (1881-end), N-GST-tagged

• Cat.No.: ROS1-24H
• Product Overview: Recombinant human ROS1 (L2086F) (1881-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1881-end
• Description: ROS1 is a proto-oncogene and member of the sevenless subfamily of tyrosine kinase insulin receptor genes. ROS1 is highly-expressed in a variety of tumor cell lines and functions as a growth or differentiation factor receptor. The FIG gene can fuse with the ROS1 gene in glioblastoma cell lines. The resulting ROS1/FIG fusion protein is a constitutively activated tyrosine kinase. Direct interaction of ROS1 and the phosphatase SHP-1 can lead to efficient downregulation of ROS1-mediated signaling. Binding sites in the ROS1 cytoplasmic domain display high affinity binding to the SHP-1 N-terminal SH2 domain. Structural models of ROS1 (L2086F) predict resistance to crizotinib, entrectinib, and lolartanib via steric hindrance.
• Bio-activity: The specific activity of ROS1(L2086F) was determined to be 121 nmol/min/mg as per actity assay protocol.
• Molecular Mass: Observed MW: ~82 kDa.
  Calculated MW: ~79 kDa.
• Purity: The purity of ROS1(L2086F) was determined to be > 90 % by densitometry.
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, and 25% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: ROS1 c-ros oncogene 1, receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: ROS1
• Synonyms: ROS1; c-ros oncogene 1, receptor tyrosine kinase; v ros avian UR2 sarcoma virus oncogene homolog 1, v ros UR2 sarcoma virus oncogene homolog 1 (avian); proto-oncogene tyrosine-protein kinase ROS; c ros 1; MCF3; ROS; proto-oncogene c-Ros; proto-oncogene c-Ros-1; c-Ros receptor tyrosine kinase; receptor tyrosine kinase c-ros oncogene 1; v-ros UR2 sarcoma virus oncogene homolog 1; transmembrane tyrosine-specific protein kinase; v-ros avian UR2 sarcoma virus oncogene homolog 1; c-ros-1
• Gene ID: 6098
• mRNA Refseq: NM_002944
• Protein Refseq: NP_002935
• MIM: 165020
• UniProt ID: P08922