Recombinant Acetobacter Pasteurianus APALIM Protein (1-429 aa), His-tagged

• Cat.No.: APALIM-1811A
• Product Overview: Recombinant Acetobacter Pasteurianus (Acetobacter turbidans) APALIM Protein (1-429 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Microbiology. Protein Description: Full Length.

Specification

• Species: Acetobacter Pasteurianus
• Source: Yeast
• Tag: His
• Protein Length: 1-429 aa
• Description: This methylase recognizes the double-stranded sequence GTGCAC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the ApaLI endonuclease.
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 48.5 kDa
• AA Sequence: MNKDEVVVSLFAGAGGFSSGFSQAGLKPLFGAEINADACQTYQENVGSPCHQLDLSTVDPSHIEMLTGGKRPFVVIGGPPCQGFSTAGPRNFADPRNLLIFNYLNIVERLSPRWLIFENVEGLLTSGGGRDLARLVREFVDMGYSVRLQKVNLAAYGVPQTRKRVLIIGNRLGIDFQFPEELYSFDSGKAKKASGKPLAPSLAEAVAGLGPAASDKDALVPYASSEPVNAFDARMRAGNRVEVVTHHVRVEAAERMQVELLKPGQTMKDLPPELWHESYRRRANRRVSDGTPTEKRGGAPSGIKRLHGNLQSLTITGPAAREFIHPTEHRPLTIRECARIQTFPDKYRWVGNNASVIQQIGNAVPPLAAERLAKHLRDIDGSFGADTRPAGAMSAKLLGFVLTEALGMSPALKSTEALLAEMHQGGFVF
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Synonyms: apaLIM; Cytosine-specific methyltransferase ApaLI
• UniProt ID: O52702