| Species : |
Mouse |
| Source : |
Rabbit |
| Tag : |
Non |
| Antigen Description : |
Cathepsin D (CTSD) is a well known lysosomal aspartyl protease and is a member of the peptidase C1 family found in almost all cells and tissues of mammals. It has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins, the generation of bioactive proteins, antigen processing, etc. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis, and can also induce apoptosis in presence of cytotoxic factors. However, on contrast, in certain studies an inhibitory role of CTSD in apoptosis was also reviewed. In addition, CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases. |
| Specificity : |
Mouse Cathepsin D / CTSD. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA. |
| Immunogen : |
Recombinant Mouse Cathepsin D / CTSD Protein |
| Isotype : |
Rabbit IgG |
| Clone : |
A103 |
| Applications : |
WB; ELISA |
| Dilution : |
Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse CTSD in WB. Using a DAB detection system, the detection limit for Mouse CTSD is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CTSD. The detection limit for Mouse CTSD is approximately 0.00975 ng/well. |
| Preparation : |
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Mouse Cathepsin D / CTSD |
| Format : |
0.2 μm filtered solution in PBS with 5% trehalose |
| Storage : |
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
