Rabbit Anti-Human CD4 Monoclonal Antibody

• Cat.No.: CABTT-005RH

Specification

• Species: Human
• Source: Rabbit
• Tag: Non
• ProductOverview: Monoclonal Antibody toCD4; Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
• Antigen Description: This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
• Clone: FQS7277
• Host animal: Rabbit
• CrossReactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant fragment corresponding to the extracellular domain of Human CD4
• Positive control: Human fetal thymus and Human lymph node lysates, THP-1 cells
• Application: WB; ICC/IF; Flow Cyt
• Sequencesimilarities: Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
• Post-translationalmodifications: Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
• Cellular localization: Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
• Form: Liquid
• Purity: Tissue culture supernatant
• Storage Buffer: pH: 7.40; Preservative: 0.01% Sodium azide; Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
• Storage: Store at -20°C. Stable for 12 months at -20°C
• Function: MHC class II protein binding; coreceptor activity; enzyme binding; extracellular matrix structural constituent; glycoprotein binding; protein binding; protein homodimerization activity; protein kinase binding; receptor activity; transmembrane signaling receptor activity; zinc ion binding

Gene Information

• Gene Name: CD4 CD4 molecule [ Homo sapiens ]
• Official Symbol: CD4
• Synonyms: CD4; CD4 molecule; CD4mut; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD 4; CD4 (L3T4); CD4 antigen; CD4+ Lymphocyte deficiency; CD4_HUMAN; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; OTTHUMP00000238897; p55; T cell antigen T4; T cell differentiation antigen L3T4; T cell OKT4 deficiency; T cell surface antigen T4/Leu 3; cell surface glycoprotein CD4; T cell surface antigen T4/Leu 3; W3/25; W3/25 antigen
• Gene ID: 920
• mRNA Refseq: NM_000616
• Protein Refseq: NP_000607
• MIM: 186940
• UniProt ID: P01730
• Chromosome Location: 12p13.31
• Pathway: Adaptive Immune System; Alpha-defensins; Antigen processing and presentation; Arf1 pathway, organism-specific; Binding and entry of HIV virion; CXCR4-mediated signaling events; Cell adhesion molecules (CAMs); Defensins; Downstream TCR signaling; Nef Mediated CD4 Down-regulation