Rabbit Anti-Human CD27 Molecule Monoclonal Antibody, FITC-conjugated

• Cat.No.: CAB11495RH
• Product Overview: Rabbit monoclonal antibody to human CD27 molecule, FITC-conjugated.

Specification

• Antigen Description: CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, and during which, adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling processes. In addition, a proapoptotic protein named SIVA, is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis.
• Specificity: Human CD27 / TNFRSF7, Has cross-reactivity in ELISA with, Mouse CD27, No cross-reactivity in ELISA with, Human TNFRSF8/CD30, Human 4-1BB, Human CD40, Human DR6, Human FAS, Human HVEM/TNFRSF14, Human TNFRI, Human TNFRII, Human BAFF, Human RELT, Mouse HVEM, Mouse FAS,Rat FAS.
• Immunogen: Recombinant human CD27 / TNFRSF7 protein
• Host: Rabbit
• Isotype: Rabbit IgG
• Species: Human
• Clone: 024
• Conjugation: FITC
• Applications: Western Blot; Flow Cytometry
• Dilution: Flow Cytometry: Human lymphocytes were stained with anti human CD27-CAB11512 (solid line) or rabbit IgG isotype control (dashed line).Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human CD27 in WB. Use of this antibody under reducing conditions is not recommended.
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CD27 and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
• Format: Aqueous solution containing 0.5% BSA and 0.1% sodium azide
• Concentration: 5 μl/Test, 0.2 mg/ml
• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze!Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
• Tag: Non

Gene Information

• Gene Name: CD27 CD27 molecule [ Homo sapiens ]
• Official Symbol: CD27
• Synonyms: CD27; CD27 molecule; TNFRSF7,tumor necrosis factor receptor superfamily, member 7; CD27 antigen; S152; Tp55; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; T14; TNFRSF7; MGC20393
• Gene ID: 939
• mRNA Refseq: NM_001242
• Protein Refseq: NP_001233
• MIM: 186711
• UniProt ID: P26842
• Chromosome Location: 12p13
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem
• Function: cysteine-type endopeptidase inhibitor activity involved in apoptotic process; protein binding; receptor activity; transmembrane signaling receptor activity