Specification
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Species :

Microorganism

Source :

Microorganism

Tag :

Non

Description :

Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP.

Predicted MolecularMass :

68kDa

Isoelectic point :

5.2

Michaelis constant :

1.1mM (ADP); 2.2mM(PEP)

Optimum pH :

7.5

Optimum temperature :

80°C

pH Stability :

5.0~10.0 (37°C, 20hr)

Thermal Stability :

< 60°C (pH8.5, 20min)

Inhibitors :

Ag+, Hg2+

Specific Activity :

≥ 300U/mg protein

Unit definition :

One unit will convert one micromole of phosphoenolpyruvate to pyruvate per min at pH 7.2 at 30°C.

Storage :

-20°C

PreparationInstructions :

The enzyme is reconstituted in 50mM Tris-HCl buffer, pH 8.5 for activity assay.

Background :

Introduction

The protein is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).

Keywords

Pyruvate kinase; EC: 2.7.1.40; CTHBP; Cytosolic thyroid hormone binding protein; MGC3932; OIP 3; Oip3; OPA interacting protein 3; Opa interactivity protein; PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase isozyme R/L; Pyruvate kinase liver and RBC; Pyruvate kinase muscle isozyme; R type/L type pyruvate kinase; RPK; THBP 1; Thyroid hormone binding protein cytosolic