PRMT3 Homogeneous Assay Kit

• Cat.No.: Kit-1856
• Product Overview: PRMT3 is a histone methyltransferase that exhibits methylation activity toward H4-R3.

Specification

• Tag: Non
• Size: 384 reactions
• Description: The PRMT3 Homogeneous Assay Kit is designed to measure the activity of PRMT3 for screening and profiling applications, using AlphaLISA technology. The key to the PRMT3 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT3 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
• Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
• Storage: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Kit Components: PRMT3: 10 µg; -80°C 100 µM S-adenosylmethionine: 500 μl; -80°CPrimary antibody 4: 400 μl; -80°CBiotinylated histone H4 peptide substrate: 400 μl; -80°C 4x HMT assay Buffer 2: 3 ml; -20°C 4x Detection buffer: 2 ml; -20°C