Phospholipase D Assay Kit

• Cat.No.: Kit-0704
• Product Overview: Phospholipase D Assay Kit is a quantitative colorimetric/fluorimetric phospholipase D activity determination.

Specification

• Tag: Non
• Description: PHOSPHOLIPASE D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers.
• Applications: Direct Assays: phospholipase D in biological samples.Drug Discovery/Pharmacology: effects of drugs on phospholipase D metabolism.
• Usage: For research use only (RUO)
• Storage: Store all components at -20°C. Shelf life of six months after receipt.
• Kit Components: Assay Buffer 10 mLDye Reagent 120 µLEnzyme Mix (Dried) 1 vialSubstrate 1.5 mLCalibrator 400 µL
• Detection method: Colorimetric, Fluorometric
• Compatible Sample Types: Biological Sample
• Features & Benefits: Sensitive. Use 10 µL samples. Detection range: colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L.Simple and High-throughput: the assay involves addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.