PCSK9[Biotinylated]-LDLR Binding Assay Kit

• Cat.No.: Kit-1807
• Product Overview: PCSK9 is a crucial player in the regulation of plasma cholesterol homeostasis. It binds to the ectodomain of hepatic low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation.

Specification

• Tag: Non
• Size: 96 reactions
• Description: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
• Applications: Great for screening small molecules and antibodies that inhibit the binding of PCSK9 to LDLR ectodomain.
• Storage: Mixed storage conditions. Stable at least 12 months from date of receipt, when stored as directed
• Kit Components: PCSK9, Biotinylated: 10 µg; -80°C LDLR: 10 µg; -80°C Streptavidin-HRP: 15 µl; +4°C 3x PCSK9 assay buffer: 50 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate (2 components): 6 ml; each +4°C White 96-well microplate: 1; +4°C