PC-3 Whole Cell Lysate
Cat.No. : | PC-3-173H |
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Species : | Human |
Tag : | Non |
Form : | The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Concentration : | 2.0 mg/ml |
Cell line : | PC-3 (Human Prostate Adenocarcinoma) |
Growth media : | DMEM/Hams F12 & 10% NCS (newborn Calf serum) |
Preparation method : | PC-3 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. |
Expiration Date : | One year from date of receipt |
Size : | 0.1 mg, 0.5 mg |
Storage Instruction : | This whole cell lysate is stable for three months at -20°C |
Applications : | This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
◆ Cell & Tissue Lysates | ||
PC-3-058WCY | Human Prostate Adenocarcinoma PC-3 Whole Cell Lysate | +Inquiry |
PC-3-01HL | Human PC-3 lysate | +Inquiry |
PC-3-173H | PC-3 Whole Cell Lysate | +Inquiry |
PC-3-015HCL | Human PC-3 Whole Cell Lysate | +Inquiry |
Not For Human Consumption!
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