NHS Activated Agarose High Flow

• Cat.No.: NHS-001A
• Product Overview: NHS Actived Agarose High Flow is a recently developed pre-activated agarose matrix that increases the choice of coupling chemistries available to these users. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. NHS Actived Agarose High Flow provides a spacer arm and is therefore particularly suitable for immobilizing small protein and peptide ligands.

Specification

• Tag: Non
• Cat.no: NHS-001A
• Size: 25 mL, 500 mL, 5 L
• Matrix: Highly cross-linked 4% agarose, spherical
• Functional group: N-hydroxysuccinimide (NHS)
• Average particle size: 90 μm
• Ligand: N-hydroxysuccinimide (NHS) functional groups
• Dynamic binding capacity: >30mg/mL (slurry) or >25mg/mL (dry resin)
• Recommended flow rate: 150cm/h at 100 kPa
• Viscosity: Before storage we recommend to wash the column volumns with of 20% ethanol to prevent microbial growth. Store the column in 20% ethanol at +4-8°C.
• Recommended column height: 5‐20cm
• pH working range: 3-13
• pH CIP range: 2-13
• Temperature Stability: 2‐40 °C
• Elution buffer: 0.1 M glycine-HCl, pH 2.7