Native Saponaria officinalis ribosome-inactivating Protein

• Cat.No.: Saporin-30S
• Product Overview: Saporin is purified from the seeds of the Soapwort plant (Saponaria officinalis). The product is routinely tested for activity by a protein synthesis inhibition assay. Saporin has no known specificity.

Specification

• Species: Saponaria officinalis
• Tag: Non
• Description: Saporin is obtained from the seeds of the Soapwort plant (Saponaria officinalis), a plant that grows wildly in Britain and other parts of Europe. Saporin is a plant enzyme with N-glycosidase activity that depurinates a specific nucleotide in the ribosomal RNA 28S, thus irreversibly blocking protein synthesis. It belongs to the well-characterized family of ribosome-inactivating proteins (RIPs). There are two types of RIPs: type I (like Saporin) are much less cytotoxic due to the lack of the B chain and type II, which are distinguished from type I RIPs by the presence of the B chain and their ability to enter cells on their own. However, type I RIPs can still be internalized by fluid-phase endocytosis. Upon internalization, the ribosomes are inactivated, resulting in cell death.
• Tag: Non
• Molecular Mass: 30 kDa
• Usage: For most targeted toxins, there are control conjugates available; these are the most accurate controls to use. For a few products, saporin is used as control. Calculate amount of saporin needed on a molar basis. For example, FGF-SAP has an average molecular weight of 63 kDa, and 71% of that is saporin (approximately 1.5 molecules of saporin per molecule of cytokine). For injections of 5 μg of immunotoxin, an appropriate control quantity would be 3.6 μg.
• Applications: Saporin serves as a control for targeted saporin immunotoxins or ligand toxins.
• Storage: Gently spin down material 5-10 seconds in a microfuge before use. Aliquot and store frozen at -20 or -80 centigrade. Avoid repeated freezing and thawing. For disposal: autoclave, or expose to 0.2 M NaOH, materials that come into contact with the toxin.
• Storage Buffer: PBS (0.14 M Sodium Chloride; 0.003 M Potassium Chloride; 0.002 M Potassium Phosphate; 0.01 M Sodium Phosphate; pH 7.4), no preservative. Sterile-filtered.
• References: 1. Stirpe F, Barbieri L, Battelli MG, Soria M, Lappi DA. (1992) Ribosome-inactivating proteins from plants: present status and future prospects. Bio/Technol 10:405-412.
  2. Lappi DA, Esch FS, Barbieri L, Stirpe F, Soria M. (1985) Characterization of a Saponaria officinalis seed ribosome-inactivating protein: immunoreactivity and sequence homologies. Biochem Biophys Res Commun 129:934-942.
  3. Stirpe F, Gasperi-Campani A, Barbieri L, Falasca A, Abbondanza A, Stevens WA. (1983) Ribosome-inactivating proteins from the seeds of Saponaria officinalis L. (soapwort) of Agrostemma githago L. (corn cockle) and of Asparagus officinalis (asparagus) and from the latex of Hura crepitans L. (sandbox tree). Biochem J 216:617-625.

Gene Information

• Official Symbol: Saporin
• Synonyms: Saporin