Native Red Fluorescent Protein

• Cat.No.: R-PE-004R
• Product Overview: R-PE is a phycobiliprotein purified from Febico’s proprietary red algae. It is made up of (αβ) 6γ subunit structure.
  ABSORPTION MAXIMUM: 566 nm
  EMISSION MAXIMUM: 575 nm
  EXTINCTION COEFFICIENT: 1.96 X 106 cm^-1 M^-1
  QUANTUM YIELD: 0.84

Specification

• Species: Red algae
• Source: Red algae
• Tag: Non
• Description: R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields.
• Form: Suspension
• Molecular Mass: 240 kDa
• Purity: A566/A280 ≥ 5.5
  A566/A498 ≤1.5
• Applications: 1. Can be cross linked or conjugated to other molecules to make fluorescent probes.
  2. Useful as a fluorescence-based indicator for the presence of cyanobacteria.
  3. Can be used for a variety of immunofluorescence applications.
  4. A useful fluoroprobe in various electrophoretic procedures.
• Usage: For Research Use Only! Not to be used in humans
• Stability: The protein is very stable and can be stored in this buffer for many years.
• Storage: Store R-PE in dark at ~ 2-8 centigrade. Do not freeze
• Storage Buffer: 100 mM Potassium Phosphate buffer, pH 7.0 with 60% Saturated (NH4)2SO4, 1 mM EDTA and 1 mM Sodium Azide.
• Reconstitution: Before use, centrifuge the R-PE suspension at 10,000g for 10 min at 4 centigrade. Discard the supernatant and resuspend the pellet (R-PE) into the desired buffer. R-PE is stored in a salt solution and it must be extensively dialyzed or desalted in columns prior to use, to remove any remaining ammonium sulphate.
• Handling: Centrifuge the vial prior to opening.

Gene Information

• Synonyms: RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin