Native Rat Collagen Type I and III Protein

• Cat.No.: Collagen Type I & III-08R
• Product Overview: Purified type I & III native rat collagen purified from rat tissue. Highest quality collagen for research use.

Specification

• Species: Rat
• Source: Rat
• Description: Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
  Type III collagen is the second most abundant collagen in tissues and is found most commonly in tissues exhibiting elastic properties such as skin, lungs, intestinal walls and walls of blood vessels. It is a homotrimer comprised of three alpha-1 chains and resembles other fibrillar collagens in structure and function. It is synthesized as procollagen, similary to collagen I, but the N-terminal propeptide remains attached in the mature fibrillar type III form.
• Form: Lyophilized, salt free, 10 mg/vial
• Purity: Rat collagen type I - 90%
  Rat collagen type III - 10%
  Other collagens - below 1%
  Non-collagen proteins - below 0.5%
• Applications: Used for coating material for cell culture studies. May not be suitable for 3-D gel formation.
• Storage: Collagen dissolved in acetic acid is stable at 4 centigrade for 1 month. Lyophilized collagen long term storage (2 years) at -20 centigrade or lower.
• Reconstitution: Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils.

Gene Information

• Official Symbol: Collagen Type I and III
• Synonyms: COL1A1; COL1A2; COL3A1; Collagen type III, alpha 1; osteogenesis imperfecta; Collagen Type I and III