Native Kidney bean Phaseolus vulgaris Lectin (PHA-E), Texas Red-Conjugated

• Cat.No.: PHA-E-555K
• Product Overview: Native Kidney bean Phaseolus vulgaris Lectin (PHA-E) - Texas Red
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: Complex N-Glycans
  Inhibiting or Eluting Sugar: Bovine Thyroglobulin, Acetic Acid
  Divalent Ions: Ca++, Mn++
  Excitation: 595 nm
  Emission: 615 nm
  Mitogenic Activity: Yes
  Hazardous Shipping: Non-hazardous
  Fluorescence: Red

Specification

• Description: Affinity purified Phaseolus vulgaris lectin (PHA-E) is tetrameric glycoprotein and responsible for the erythroagglutinating properties of the PHA fraction. It has a carbohydrate specificity towards oligosaccharides and elutes with bovine thyroglobulin or acetic acid. PHA-E will bind to both human erythrocytes and lymphocytes, with a specificity towards blood group A (-SA). There are five times more PHA-E receptors on normal human lymphocytes than there are on erythrocytes. This lectin is widely used as a biochemical tool for detecting bisecting GlcNAc- and Gal-bearing glycoproteins. Texas Red is a red-fluorescent dye and when bound to Phaseolus vulgaris Lectin (PHA-E) can show the binding pattern of this lectin in cellular imaging applications. There is very little overlap between the emission spectra of Texas Red and FITC making this combination ideal for dual-labeling experiments. Rhodamine dyes, such as Texas Red, are more photostable and less sensitive to pH change when compared to other dyes such as fluorescein.
• Source: Kidney bean
• Species: Kidney bean
• Form: Liquid
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: At -20 centigrade.
• Concentration: 2 mg/mL

Gene Information

• Official Symbol: PHA-E
• Synonyms: Phaseolus vulgaris Lectin; PHA-E)