Native Kidney bean Phaseolus vulgaris Lectin (PHA-E+L), FITC-Conjugated

• Cat.No.: PHA-E+L-322K
• Product Overview: Native Kidney bean Phaseolus vulgaris Lectin (PHA-E+L) - FITC (Fluorescein)
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: Complex N-Glycans
  Inhibiting or Eluting Sugar: Bovine Thyroglobulin, Acetic Acid
  Divalent Ions: Ca++, Mn++
  Excitation: 495 nm
  Emission: 515 nm
  Mitogenic Activity: Yes
  Hazardous Shipping: Non-hazardous
  Fluorescence: Green

Specification

• Description: Affinity Purified Phaseolus vulgaris lectin (PHA E+L, also known as PHA P, P standing for phytohemagglutinin) is affinity purified lectin consists of two isolectins PHA-E and PHA-L, erythroagglutinin and leucoagglutinin, of which both possess strong mitogenic activity. PHA P has a molecular weight of 126,000 and an isoelectric point between pH 5. 2 and pH 6. 2. It has a carbohydrate specificity for complex oligosaccharides and elutes with bovine thyroglobulin or acetic acid. This lectin agglutinates blood group A (-SA). PHA P can be used for mitogenic stimulation to human lymphocytes. It has the potential to induce closer contacts between adjacent cell membranes and has been successfully used for membrane-induced fusion in human oocytes, bovine oocyt, and caprine oocytes. Phytohemagglutinin was direct evidence for the involvement of bacterial lectins in the initiation of infection, the basis for present attempts in academia and industry to apply carbohydrates for anti-adhesion therapy of such diseases. Phaseolus vulgaris Lectin (PHA-E+L) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications.
• Source: Kidney bean
• Species: Kidney bean
• Form: Liquid
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: At -20 centigrade.
• Concentration: 2 mg/mL

Gene Information

• Official Symbol: PHA-E+L
• Synonyms: Phaseolus vulgaris Lectin; PHA-E+L