Native Human Proteasome 26S Protein, Tag Free

• Cat.No.: Proteasome 26S-38H
• Product Overview: Purified from human blood cells by fractination. The highest activity, soluble and homogenous. Consists of a high purity mixture of '26S' proteasomes singly (26S) and doubly (30S) capped with 19S regulatory subunit complexes in the ratio of 40 % single cap : 60 % double capped at the time of preparation.

Specification

• Species: Human
• Source: Blood cell
• Tag: Non
• Description: The proteasome is a multisubunit enzyme complex that plays a central role in the regulation of proteins that control cell-cycle progression and apoptosis, and has therefore become an important target for anticancer therapy.
• Tag: Non
• Applications: A highly purified preparation of '26S' proteasomes, useful for carrying out in vitro protein degradation studies with suitably ubiquitinylated protein substrates.
• Stability: When ready for use the enzyme should be thawed by standing on ice. If the enzymatic acitivity of the 26S proteasome is to be measured, it should be used immediately after thawing since the enzyme complex is labile. After dissociaton of the 26S complex the 20S proteasome activity is relatively stable.
• Storage: At -80 centigrade
• Storage Buffer: Suspended in TSD buffer (50μg in 10mM TRIS, containing 25mM potassium chloride, 1.1mM magnesium chloride, 0.1mM ethylenediaminetetraacetic acid, 1mM dithiothreitol, 1mM sodium azide, 2mM ATP, pH 7.0, and 35 % glycerol).
• Shipping: Dry ice

Gene Information

• Official Symbol: Proteasome 26s
• Synonyms: Proteasome 26S; 26S