Native Human Collagen type I Protein

• Cat.No.: Collagen type I-01H
• Product Overview: Human collagen type I protein purified from human placenta, Negative for HBsAg, HCV, HIV1 and HIV2.

Specification

• Description: Type I collagen is a fibrillar type collagen, and most likely the best investigated collagen. Type I collagen is the most abundant collagen and is the key structural composition of several tissues. It is expressed in almost all connective tissues and the predominant component of the interstitial membrane.
• Source: Placenta
• Species: Human
• Form: Lyophilized
• Purity: Human collagen type I at least 90%
  human collagen type III below 10%
  Other human collagens and non-collagen proteins below 1%
  Purity is controlled by SDS-PAGE and by ELISA with anti-collagen type-specific antibodies
• Enzyme Unit: Storage 2 years at -20 centigrade or lower
• Applications: Human collagen type I standard e.g. for ELISA and dot blotting
  Antigen for antibody production
  Coating material for cell culture studies
  Glycation of collagen type I, coating at 10 μg/cm2 and adhesion of pancreatic islet-derived mesenchymal stem cells (PI-MSCs)
  Collagen type I was used in cell substrates to various stiffness. Cell topography (HFF, human glioblastoma cell line U87, human foreskin fibroblast) depends on substrate stiffness and 3D microenvironment
  Cell attachment assay
  The zona pellucida of a blastocyst-stage human embryo was plated on 10 ng/mL collagen type I
  Collagen type I rescue experiments
• Reconstitution: Use 0.1 M acetic acid, pH 3.0
• Shipping: Shipping at ambient temperature.

Gene Information

• Official Symbol: Collagen type I
• Synonyms: COL1A1; COL1A2; Collagen type I; collagen type I alpha 2 chain; OI4; EDSCV; EDSARTH2; collagen alpha-2(I) chain; alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-2 type I collagen; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-2 chain; collagen, type I, alpha 2; epididymis secretory sperm binding protein; type I procollagen; collagen type I alpha 1 chain; OI1; OI2; OI3; OI4; EDSC; CAFYD; EDSARTH1; collagen alpha-1(I) chain; alpha-1 type I collagen; alpha1(I) procollagen; collagen alpha 1 chain type I; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; pro-alpha-1 collagen type 1; type I proalpha 1; type I procollagen alpha 1 chain