Native Carica papaya latex Papain

• Cat.No.: Papain-12
• Product Overview: Native Carica papaya latex Papain

Specification

• Species: Carica papaya
• Stability: Stable for ~ 6months at 2-8 centigrade. Do not freeze aqueous suspensions.
• Applications: Cell isolation where it is more gentle than other proteases (i.e.: cortical neurons, retina, and smooth muscle) Protein structural studies, peptide mapping Red cell surface modification for antibody screening or identification Fab preparation from IgG and IgM antibodies Solubilization of integral membrane proteins Production of glycopeptides from purified proteoglycans Enzymatic wound debridement
• Notes: Papain preparations should be incubated in the activation solution before use to ensure full activity. Applications include antibody fragmentation and primary/neural cell isolation.
• Reference: 1. Akahane, K. and Umeyama, H. Binding Specificity of Papain and Cathepsin B , Enzyme 36 , 141 , 1986 2. Alecio, M. , Dann, M. and Lowe, G. The Specificity of the S1 Subsite of Papain , Biochem J 141 , 495 , 1974 3. Allen, G. and Lowe, G. Investigation of the Active Site of Papain with Fluorescent Probes , Biochem J 133 , 679 , 1973

Gene Information

• Synonyms: Papain; Car p 1; EC:3.4.22.2; Papaya proteinase I; PPI
• UniProt ID: P00784