Native Bovine Anhydrotrypsin

• Cat.No.: LOC780933-1B

Specification

• Species: Bovine
• Source: Bovine pancreas
• Tag: Non
• Description: Treatment of trypsin with phenylmethylsulfonyl fluoride (PMSF) followed by a strong base leads to the conversion of Serine 195 to dehydroalanine at the active site. Alpha and beta forms of anhydrotrypsin are separated by immobilized soybean trypsin inhibitor. The activity of anhydrotrypsin is greatly reduced compared to native trypsin as quantified by chromogenic substrate. Anhydrotrypsin provided by our company is the single chain beta-form prepared from Type XIII bovine trypsin.
• Form: Frozen liquid
• Molecular Mass: 23,300
• Purity: >95% by SDS-PAGE analysis
• Usage: For in vitro laboratory use only
• Notes: Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
• Storage: -70°C
• Concentration: 2.0 mg/ml
• Storage Buffer: 1mM HCl; 10mM CaCl2
• Shipping: Dry ice

Gene Information

• Gene Name: LOC780933 cationic trypsin [ Bos taurus ]
• Official Symbol: LOC780933
• Synonyms: cationic trypsinbeta-trypsin; trypsin, beta
• Gene ID: 780933
• mRNA Refseq: NM_001113727
• Protein Refseq: NP_001107199
• UniProt ID: P00760
• Chromosome Location: chromosome: 4
• Pathway: Influenza A, organism-specific biosystem; Influenza A, conserved biosystem; Neuroactive ligand-receptor interaction, conserved biosystem
• Function: metal ion binding; protein binding; serine-type endopeptidase activity