Native Bovine ACTC1 Protein, Pyrene labeled

• Cat.No.: ACTC1-885B
• Product Overview: Native Bovine ACTC1 with a pyrenyl group covalently bound to Cys374 at the C-term of the molecule was purified from Bovine cardiac.

Specification

• Species: Bovine
• Source: Bovine cardiac
• Tag: Non
• Description: G-actin from cardiac muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. Pyrene actin is chemically modified G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the molecule. Pyrene Actin (10%) contains 10% of pyrenyl actin with a dye/protein molar ratio of 1.0.
• Form: 2mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution.
• Molecular Mass: 42kDa
• Purity: >99% by scanning densitometry.
• Applications: Fluorimetrical Analysis of actin dynamics (e.g. kinetics of polymerization, nucleation, depolymerization)
• Storage: Pyrene actin is stored at –70 centigrade upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week. Avoid refreezing.
• Shipping: At ambient temperature. Upon delivery store at -70 centigrade.

Gene Information

• Gene Name: ACTC1 actin, alpha, cardiac muscle 1 [ Bos taurus ]
• Official Symbol: ACTC1
• Synonyms: ACTC1; actin, alpha, cardiac muscle 1
• Gene ID: 533219
• mRNA Refseq: NM_001034585
• Protein Refseq: NP_001029757
• UniProt ID: Q3ZC07