Mouse Anti-Virus HBsAg Hybridoma

• Cat.No.: CSC-H0153

Specification

• Cell Line Description: The antibody is directed against hepatitis B surface antigen.
• Introduction: Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3. 2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBxprotein. HBxhas been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBxis believed to exert transcriptional activity through protein protein interaction.
• Immunogen: Hepatitis B virus surface antigen
• Immunological Donor: Mouse spleen
• Fusion Species: Mouse X Mouse Hybridoma
• Myeloma: NS1
• Growth properties: Suspension
• Propagation: Complete growth medium: DMEM + 2mM glutamine + 15% Foetal Bovine Serum (FBS) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C
• Subculturing: Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C.
• Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
• Reactivity: Anti-hepatitis B virus surface antigen
• Isotype: IgG1
• Application: Immunochiognosis
• Storage: Liquid nitrogen
• Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
• Ship: Dry ice
• Tag: Non

Q&As

What size is the recombinant HBsAg? Is it the L, M or S version of the antigen?

The HBsAg proteins are generally provided in 100ug, 500ug, and 1mg packing sizes. We can also aliquot based on your specific requirements. Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. As the sequence of the below antigens is confidential, we cannot tell that if they are L, M, or S versions.