Mouse Anti-Sheep CD4 Hybridoma

• Cat.No.: CSC-H0008

Specification

• Species: Sheep
• Tag: Non
• Background: CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene.
• Cell Line Description: The antibody is directed against the CD4 molecule of sheep and goats.
• Depositor: Dr C Mackay, Basel Institute for Immunology, Basel
• Immunogen: Sheep lymphocytes
• Immunological Donor: BALB/c mouse spleen
• Fusion Species: Mouse X Mouse Hybridoma
• Myeloma: Sp2/0
• Growth properties: Suspension
• Morphology: Lymphoblast
• Propagation: Complete growth medium: DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C
• Subculturing: Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C.
• Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
• Reactivity: Anti-CD4, ovine
• Isotype: IgG3
• Target: CD4
• Storage: Liquid nitrogen
• Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
• Ship: Dry ice

Gene Information

• Gene Name: CD4 CD4 molecule [ Ovis aries ]
• Official Symbol: CD4
• Synonyms: CD 4; CD4 (L3T4); CD4; CD4 antigen (p55); CD4 Antigen; CD4 molecule; CD4 Receptor; CD4+ Lymphocyte deficiency; CD4_HUMAN; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; OTTHUMP00000238897; p55; T Cell Antigen T4; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3; T cell surface antigen T4/Leu3; T Cell Surface Antigen T4/Leu3; T Cell Surface Glycoprotein CD4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; W3/25; W3/25 antigen
• Gene ID: 443509
• mRNA Refseq: NM_001129902
• Protein Refseq: NP_001123374