Mouse Anti-Human CD244 Molecule, Natural Killer Cell Receptor 2B4 Monoclonal Antibody

• Cat.No.: CAB11493MH
• Product Overview: Mouse monoclonal antibody to human CD244 molecule, natural killer cell receptor 2B4.

Specification

• Species: Human
• Source: Mouse
• Tag: Non
• Antigen Description: The CD244 antigen, also known as 2B4 / CD244, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. The 2B4 / CD244 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 / CD244 can effectively activate and enhance NK cell –mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a member of the CD2 subset of the Ig superfamily, CD244 is a high affinity ligand for CD48 and the interaction is essential for generation of NK effectors.
• Specificity: Human 2B4 / SLAMF4 / CD244. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA. Human SLAMF1, Human cell lysate (293 cell line).
• Immunogen: Recombinant Human 2B4 / SLAMF4 / CD244 Protein
• Isotype: Mouse IgG1
• Clone: 4G4B7
• Purification: Protein A affinity chromatography
• Applications: ELISA
• Dilution: Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect Human 2B4. The detection limit for Human 2B4 is approximately 0.039 ng/well.
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human 2B4 / SLAMF4 / CD244. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: CD244 CD244 molecule, natural killer cell receptor 2B4 [ Homo sapiens ]
• Official Symbol: CD244
• Synonyms: CD244; CD244 molecule, natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4 ,natural killer cell receptor 2B4; natural killer cell receptor 2B4; 2B4; NAIL; NKR2B4; Nmrk; SLAMF4; h2B4; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NK cell activation inducing ligand NAIL
• Gene ID: 51744
• mRNA Refseq: NM_001166663
• Protein Refseq: NP_001160135
• MIM: 605554
• UniProt ID: Q9BZW8
• Chromosome Location: 1q23.1
• Pathway: Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem; Natural killer cell mediated cytotoxicity, organism-specific biosystem; Natural killer cell mediated cytotoxicity, conserved biosystem
• Function: protein binding; receptor activity