Active Recombinant Cynomolgus/Rhesus macaque CD244 protein, His-Avi-tagged, Biotinylated

• Cat.No.: CD244-2848C
• Product Overview: Biotinylated Recombinant Cynomolgus/Rhesus macaque CD244 protein(Leu 24-Gln 186), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: Avi&His
• Protein Length: Leu 24-Gln 186
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Cynomolgus 4-1BB Ligand, Fc Tag with a linear range of 0.3-2 ng/mL.Immobilized Cynomolgus 4-1BB Ligand, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag with a linear range of 0.3-20 ng/mL.Immobilized Human 4-1BB Ligand, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag with a linear range of 0.6-20 ng/mL.Immobilized Utomilumab at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag with a linear range of 0.3-10 ng/mL.
• Molecular Mass: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.The protein has a calculated MW of 21.8 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.