Mouse Anti-Bovine CD4 Hybridoma

• Cat.No.: CSC-H0173

Specification

• Species: Bovine
• Tag: Non
• Background: CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene.
• Cell Line Description: The antibody is directed against B°CD4 antigen, the bovine equivalent of human CD4 found on helper/inducer T cells.
• Depositor: Dr J Naessens, ILRAD, Nairobi, Kenya
• Immunogen: Bovine alloreactive lymphocytes
• Immunological Donor: BALB/c mouse spleen
• Fusion Species: Mouse X Mouse Hybridoma
• Myeloma: P3x63Ag8
• Growth properties: Suspension
• Propagation: Complete growth medium: RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C
• Subculturing: Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C.
• Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
• Reactivity: Anti-CD4, bovine
• Isotype: IgG2a
• Target: CD4
• Application: Enumeration of bovine lymphocyte populations
• Storage: Liquid nitrogen
• Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
• Ship: Dry ice

Gene Information

• Gene Name: CD4 CD4 molecule [ Bos taurus ]
• Official Symbol: CD4
• Synonyms: T-cell surface glycoprotein CD4; CD4 antigen; CD4
• Gene ID: 407098
• mRNA Refseq: NM_001103225
• Protein Refseq: NP_001096695
• Chromosome Location: 5
• Pathway: Antigen processing and presentation; Cell adhesion molecules (CAMs); Cytokines and Inflammatory Response (Bi°Carta); Hematopoietic cell lineage; Primary immunodeficiency; T cell receptor signaling pathway