Mouse Anti-Bovine CD2 Hybridoma

• Cat.No.: CSC-H0181

Specification

• Species: Bovine
• Tag: Non
• Background: CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.
• Cell Line Description: The antibody is directed against Bo CD2, the bovine equivalent of human CD2. It shows species cross-reactivity with goat.
• Depositor: Dr J Naessens, ILRAD, Nairobi, Kenya
• Immunogen: Bovine cytotoxic T cells
• Immunological Donor: BALB/c mouse spleen
• Fusion Species: Mouse X Mouse Hybridoma
• Myeloma: P3x63Ag8.653
• Growth properties: Suspension
• Propagation: Complete growth medium: RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C
• Subculturing: Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C. These cells may require mouse macrophage feeder cells upon resuscitation.
• Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
• Reactivity: Anti-CD2, bovine
• Isotype: IgG2a
• Target: CD2
• Application: Enumeration of bovine lymphocyte populations
• Storage: Liquid nitrogen
• Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
• Ship: Dry ice

Gene Information

• Gene Name: CD2 CD2 molecule [ Bos taurus ]
• Official Symbol: CD2
• Synonyms: T-cell surface antigen CD2; CD2 antigen (p50); sheep red blood cell receptor
• Gene ID: 497022
• mRNA Refseq: NM_001011676
• Protein Refseq: NP_001011676
• Chromosome Location: 3
• Pathway: Cell adhesion molecules (CAMs); Cell surface interactions at the vascular wall; Hematopoietic cell lineage; Hemostasis
• Function: eukaryotic cell surface binding; protein homodimerization activity; receptor activity