Recombinant Human CD2 molecule Protein, His&Flag&StrepII tagged

• Cat.No.: CD2-006H
• Product Overview: Recombinant human CD2 protein (P06729) (Lys 236-Asn 351) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Description: CD2 (also designated E-rosette receptor) interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes, as well as on mouse B cells and rat splenic macrophages. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counterreceptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.
• Source: E. coli
• Species: Human
• Tag: N-Twin strep and C-His&Flag
• Protein length: 236-351aa
• Molecular Mass: 19.5 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: CD2 CD2 molecule [ Homo sapiens (human) ]
• Official Symbol: CD2
• Synonyms: CD2; CD2 molecule; CD2 antigen (p50), sheep red blood cell receptor, SRBC; T-cell surface antigen CD2; LFA-3 receptor; rosette receptor; erythrocyte receptor; lymphocyte-function antigen-2; T-cell surface antigen T11/Leu-5; CD2 antigen (p50), sheep red blood cell receptor; T11; SRBC; LFA-2; FLJ46032
• Gene ID: 914
• mRNA Refseq: NM_001767
• Protein Refseq: NP_001758
• MIM: 186990
• UniProt ID: P06729