MMP-3 Activity Fluorometric Assay Kit

• Cat.No.: Kit-2213
• Product Overview: The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development. In MMP-3 Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em = 325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is < 50 µU. This kit can be used with our MMP-3 inhibitor, GM6001 as a control. In addition, we also offer a human recombinant MMP-3 enzyme and a MMP-3 inhibitor Screening Kit, separately.

Specification

• Applications: The assay sensitivity is < 50 µU.
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: MMP-3 Assay Buffer; MMP-3 Substrate; Mca Standard (1mM); MMP-3 Positive Control
• Target Species: Mammalian
• Detection method: Fluorescence (Ex/Em 325/393 nm)
• Features & Benefits: Sensitive assays for measuring MMP-3 in various biological samples
• Tag: Non