MBP CoIP Agarose beads, kit

• Cat.No.: MBP-02A
• Product Overview: The MBP-Trap Agarose Kit contains MBP-Trap Agarose, lysis, wash, and elution buffers for efficientImmunoprecipitation of MBP-fusion proteins and their interacting factors.
  •Immunoprecipitation of low abundant MBP-fusion proteins
  • Significantly higher sensitivity than amylose resin and other affinity resins
  • No heavy & light antibody chains
  • Stable under harsh washing conditions
  • Suitable for downstream mass spec analysis

Specification

• Specificity/Target: Maltose-binding protein (MBP) of Escherichia coli
• Binding capacity: 45 μg (0.625 nmol) of recombinant MBP-tagged protein (~74.2 kDa) per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 μm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 4 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP