Lactate Dehydrogenase Activity Assay Kit

• Cat.No.: Kit-2257
• Product Overview: Lactate Dehydrogenase (LDH: EC 1.1.1.27) is a common enzyme found in wide range of organisms. It catalyzes the interconversion of pyruvate and lactate, with concomitant interconversion of NADH and NAD+. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries. Lactate Dehydrogenase Activity Assay kit provides a quick and easy method for monitoring Lactate Dehydrogenase activity in a wide variety of samples. In this assay, Lactate Dehydrogenase converts lactate into pyruvate and NADH, which reacts with to generate an intense fluorescent product (Ex/Em = 535/587 nm). This kit is simple, highly sensitive and high-throughput adaptable, and can detect Lactate Dehydrogenase activity as low as 1 μU/ml.

Specification

• Applications: Measurement of LDH activity in various tissues and cellsEvaluation of tissue damages or cell cytotoxicity.
• Storage: -20°C
• Shipping: Gel Pack
• Size: 500 assays
• Kit Components: LDH Assay Buffer; LDH Substrate Mix (Lyophilized); PicoProbe (in DMSO); NADH Standard (Lyophilized); LDH Positive Control
• Detection method: Fluorescence (Ex/Em = 535/587 nm)
• Features & Benefits: Simple & highly sensitive;
  High-throughput adaptable;
  Detection Limit: 1 μU/ml
• Tag: Non