IWP-2, GMP Grade

• Cat.No.: IWP-2-08
• Product Overview: IWP-2 (GMP) is IWP-2 produced by using GMP guidelines. GMP small molecules work appropriately as an auxiliary reagent for cell therapy manufacture.

Specification

• Description: IWP-2 is an inhibitor of Wnt processing and secretion with an IC50 of 27 nM. IWP-2 targets the membrane-bound O-acyltransferase porcupine (Porcn) and blocks Wnt ligand palmitoylation.
• Form: Solid, White to off-white
• Target: Wnt
• In Vitro: IWP-2 (GMP) (2 μM, in the first 4 days of Stage IV induction medium) reprograms human somatic cells to pluripotent stem cells. IWP-2 (GMP) (5 μM, day 3 to 5) induces cardiac differentiation of hiPSCs. IWP-2 (GMP) (5 μM, day 1 to 3) increasing the expression of cardiac progenitors and cardiac genes (MYL2, TNNI3, and TNNT2) in hiPSCs. IWP-2 (GMP) (5 μM, day 5-7) together with PIP-S2 induces cardiac mesoderm differentiates into functional cardiomyocytes. IWP-2 (GMP) (5 μM, treated at day 3) induces cardiomyocyte differentiation when applied following a pretreatment with Laduviglusib (GMP). RT-PCR Cell Line: hiPSCs Concentration: 5 μM Incubation Time: day 1 to 3 Result: Reduced the expression of anti-cardiac mesoderm genes, and increased the expression of cardiac progenitors and cardiac genes (MYL2, TNNI3, and TNNT2)
• Solubility Data: DMSO: 2 mg/mL (4.29 mM; Need ultrasonic and warming)
• IC50 & Target: IC50: 27 nM (Wnt)
• CAS: 686770-61-6
• Formula: C22H18N4O2S3
• Molecular Mass: 466.6
• Storage: 4 centigrade, sealed storage, away from moisture

Gene Information

• Pathway: Stem Cell/Wnt
• Official Symbol: IWP-2
• Synonyms: IWP-2