Human Melanoma Lysate

• Cat.No.: Melanoma-342H

Specification

• Species: Human
• Source: Human Skin
• Tag: Non
• Concentration: 2 mg/mL or better
• Tissue Type: Skin
• Shipping: Dry ice
• Preparation method: Melanoma lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The human melanoma tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the melanoma tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The melanoma tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
• Diagnosis: Tumor
• Storage Buffer: HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
• Storage Instruction: Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
• Applications: Melanoma lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.