Human MAPK1 (ERK2) Knockout A549 Cell Lysate, Clone 15

• Cat.No.: A549-019HCL

Specification

• Species: Human
• Description: ERK2 antibodies detect the ERK2 isoform. Mitogen activated protein kinase 1, also known as MAPK1, ERK, or ERK2, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. ERK1 and ERK2 are activated by MEK1 and MEK2 in the B-raf signaling pathway resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Human ERK1 and ERK2 are 84% identical in sequence and share common functionality in cells.
• Form: Liquid (sterile filtered)
• Specificity: MAPK1 (ERK2) knockout A549 cells were grown in medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 μM Aprotinin, 5 μM Bestatin, 1.5 μM E-64, 2 μM Leupeptin Hemisulfate, 1 μM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by BCA using a commercially available kit. Protein concentration was adjusted to 2 mg/mL with modified 1× RIPA buffer. MAPK1 (ERK2) knockout A549 Clone 15 contains knockout deletions on all three copies of the MAPK1 (ERK2) gene in A549 cells. Each copy contains the same 104 bp deletion induced by CRISPR/Cas9. The deletion occurs in exon 2 and disrupts the sequence between amino acids 59 to 94. These mutations induce a frame-shift and result in early stop codons. Validated by Sanger sequencing and Western blot.
• Applications: WB, SDS-PAGE
• Cell Line: Human A549 (lung epithelial carcinoma)
• Lysate Fractionation: Whole Cell Lysate
• Lysate Stimulation: Not Stimulated
• Culture Type: Tissue Culture
• Induction: None (Control)
• Concentration: 2.0 mg/mL by BCA assay
• Buffer: 1× RIPA Buffer with HALT Protease and Phosphatase Inhibitors
• Preservative: None
• Stabilizer: None
• Shipping: Dry Ice
• Storage: Store vial at -70 centigrade or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
• Expiration: Expiration date is one year from date of receipt.

Gene Information

• Gene Name: MAPK1 mitogen-activated protein kinase 1 [ Homo sapiens (human) ]
• Official Symbol: MAPK1
• Synonyms: MAPK1; mitogen-activated protein kinase 1; ERK; p38; p40; p41; ERK2; ERT1; NS13; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; mitogen-activated protein kinase 1; MAP kinase 1; MAP kinase 2; MAPK 2; extracellular signal-regulated kinase 2; mitogen-activated protein kinase 2; protein tyrosine kinase ERK2; EC 2.7.11.24
• Gene ID: 5594
• mRNA Refseq: NM_138957
• Protein Refseq: NP_620407
• MIM: 176948
• UniProt ID: P28482