Human HCT-116 Cell Nuclear Extract

• Cat.No.: HCT-116-031HCL
• Product Overview: Multi-purpose HCT-116 nuclear extracts are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels.

Specification

• Species: Human
• Form: Liquid (sterile filtered)
• Specificity: HCT-116 cells were grown in McCoy''s medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 μM Aprotinin, 5 μM Bestatin, 1.5 μM E-64, 2 μM Leupeptin Hemisulfate, 1 μM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 4 mg/mL in RIPA buffer containing protease and phosphatase inhibitors.
• Cell Line: Human - colorectal carcinoma
• Lysate Fractionation: Nuclear Extract
• Lysate Stimulation: Not Stimulated
• Culture Type: Tissue Culture
• Induction: None (Control)
• Concentration: 4 mg/mL by BCA assay
• Buffer: 1× RIPA Buffer with HALT Protease and Phosphatase Inhibitors
• Preservative: None
• Stabilizer: None
• Shipping: Dry Ice
• Storage: Store vial at -70 centigrade or COLDER. For extended storage, aliquot Nuclear Extract to minimize freeze/thaw cycles.
• Expiration: Expiration date is three months from date of receipt.