Human ductal breast epithelial tumor cell line T47D Whole Cell Lysate
Cat.No. : | T47D-007WCY |
Product Overview : | T47D (Human ductal breast epithelial tumor cell line) Whole Cell Lysate |
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Species : | Human |
Source : | Breast |
Description : | The T-47 line was isolated by I. Keydar from a pleural effusion obtained from a 54 year old female patient with an infiltrating ductal carcinoma of the breast. |
Applications : | IP, WB |
Stability : | The lysate is stable for at least one year from date of shipping when stored at -80 centigrade. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20 centigrade for long term storage. |
Storage : | Store the lysate at -80 centigrade and avoid repeated freeze / thaw cycles. |
Concentration : | 1 vial of 100 μg whole cell lysate in RIPA buffer. 1 vial of 250 μL 2 x SDS Sample Buffer (4% SDS, 125 mM Tris-HCl pH 6.8, 10 % Glycerol, 0.002 % Bromphenol blue, 100 mM DTT) |
Storage Buffer : | Modified RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma) and 1 mM Na3VO4) |
Shipping : | Shipped on dry ice. |
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Not For Human Consumption!
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