Human Descending Colon Membrane Lysate

• Cat.No.: Colon descending-101H

Specification

• Species: Human
• Source: Human Colon Descending Tissue
• Tag: Non
• Concentration: 2 mg/mL or better
• Tissue Type: Colon descending
• Shipping: Dry ice
• Preparation method: Human colon descending tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human colon descending tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated colon descending tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated colon descending tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
• Diagnosis: Normal
• Storage Buffer: HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
• Storage Instruction: Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
• Applications: Colon descending membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.