Human Colon Lysate, Total Protein

• Cat.No.: Colon-009H
• Product Overview: Human Colon total protein (protein lysate) is isolated from freshly harvested tissues of single healthy normal donor. Tissues are homogenized in protein lysis buffer supplemented with a cocktail of 6 mammalian protease inhibitors to minimize proteolysis, precisely quantified.

Specification

• Species: Human
• Source: Colon
• Applications: Total protein is ready for immediate use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, protein-protein interaction, and tissue specific expression. Protein can also be provided in denatured SDS Sample buffer for only Western blotting use.
• Quality Control Test: Quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE using Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody.
• Stability: Dry ice
• Storage: Stored at -80 centigrade.
• Concentration: 5 mg/mL