HCT 116 (human colorectal carcinoma) whole cell lysate

• Cat.No.: HCT-776H

Specification

• Species: Human
• Tag: Non
• Tissue Type: Colon; Colorectal Carcinoma
• Preparation method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.
• Size: 100 ul
• Recommended Usage: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Storage Buffer: In modified RIPA Lysis Buffer.
• Applications: Western Blot;Immunoprecipitation