HA Magnetic CoIP Agarose kit
Cat.No. : | HA-03A |
Product Overview : | The HA-Trap Magnetic Agarose Kit contains HA-Trap magnetic agarose, lysis, wash, and elution buffers for efficientImmunoprecipitation of HA-tagged proteins. • Fast, reliable & efficient one-stepImmunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
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Specificity/Target : | Binds specifically to the HA-tag (sequence YPYDVPDYA) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion. There is no cross-reactivity to other common peptide tags such as the His6-tag, FLAG-tag, Spot-Tag, V5-tag, Strep-tag, or C-tag (other tags not tested). Background binding to host cell proteins from a range of organisms such as human, mouse and dog cell lines or yeast and plants is low. |
Binding capacity : | 20 ug of recombinant HA-tagged protein (~30 kDa) per 25 uL bead slurry |
Conjugate : | Magnetic agarose beads; ~40 um (cross-linked 6% magnetic agarose beads) |
Elution buffer : | 2xSDS-sample buffer (Lammli) |
Wash buffer compatibility : | 2x SDS-sample buffer (Lammli) |
Type : | Nanobody |
Affinity (KD) : | 6 nM for C-terminal HA-tags and ca. 180 nM for N-terminal fusions. |
Storage Buffer : | 20% ethanol |
Applications : | IP, Co-IP |
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Not For Human Consumption!
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