Active Recombinant H1N1 (A/England/195/2009) HA Protein, His-tagged

• Cat.No.: HA-791V
• Product Overview: Recombinant Influenza A virus (A/England/195/2009 (H1N1)) hemagglutinin (ACR15621.1) (Met1-Gln529), termed as HA, was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: H1N1
• Source: Insect Cells
• Tag: His
• Protein Length: 1-529 a.a.
• Predicted N Terminal: Asp 18
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0, 5% ~ 8% trehalose and mannitol.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 1-4μg/mL for 1% GRBC.
• Molecular Mass: The recombinant virus HA consists 523 amino acids and predicts a molecular mass of 58.9 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.