GST CoIP Agarose

• Cat.No.: GST-01A
• Product Overview: Immunoprecipitation of GST-fusion proteins and their interacting factors with anti-GST Nanobody conjugated to beads.
  • Effectively immunoprecipitates low expressed GST-fusion proteins
  • Considerably higher affinity than glutathione cellulose and other affinity resins
  • Strong binding even under harsh washing conditions
  • No heavy & light chains in your downstream application

Specification

• Specificity/Target: Glutathione S-Transferase (GST) from Schistosoma japonicum
• Binding capacity: 7.5-10 μg of recombinant GST per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 μm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 1 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP
• Storage: Upon receipt store at +4°C. Do not freeze!